HHSignite (beta): Fast screening CE-MS method for bacteria through protein pattern recognition
Submitted by staff from: FDA / Office of Regulatory Affairs
- Jose Moreno, FDA/ORA
- Jose Velez, FDA/ORA
- Fernando Gonzalez, FA/ORA
- Hector Espinet, FDA/ORA
- Osvaldo Rosario, FDA/ORA
- Joseph Bloom, FDA/ORA
Project Lead’s Approving Supervisor:
Adaberto Cajibas, Supervisory Chemist
Test the use of a new technology that promises to shorten the time it takes to identify bacteria.
The conventional microbiological procedures used by the FDA are labor-intensive and time-consuming. This affects directly regulatory actions that the agency must take when a faster response is needed.
A fast screening method to detect bacteria in food samples will be developed using capillary electrophoresis (CE) coupled with mass spectrometry (MS) to develop protein pattern recognition for bacterial samples in food. The use of this method could shorten analysis time in order to expedite FDA regulatory actions.
With the fast screening method of CE-MS protein-pattern recognition, samples can be analyzed in 30 minutes to determine the presence of bacteria. The proposal tests this method to show the presence of the bacteria without sample preparation, in a fast analysis time through the protein pattern in the electropherogram.
This proposal builds on a previous effort of the CDC that successfully identified a specific type of tuberculosis. If successful, this idea might also be applied to other pathogenic organisms such as shigella, bacillus, anthrasis, and also viruses in the future.